Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar
نویسندگان
چکیده
منابع مشابه
Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar
The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months without the need for subculture and confirmed the viability of all isolates by the naked eye.
متن کاملMaintenance of Leptospira Species in Leptospira Vanaporn
Vanaporn Wuthiekanun, Premjit Amornchai, Sayan Langla, Malinee Oyuchua, Nicholas P. J. Day, Direk Limmathurotsakul Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Nuffield Department of Medicine, University of Oxford, Old Road Campus, Headington, Oxford, United Kingdom; Department of Tropical Hygiene, Faculty of Tropical Medic...
متن کاملAntimicrobial Disk Susceptibility Testing of Leptospira spp. Using Leptospira Vanaporn Wuthiekanun (LVW) Agar
Leptospira Vanaporn Wuthiekanun (LVW) agar was used to develop a disk diffusion assay for Leptospira spp. Ten pathogenic Leptospira isolates were tested, all of which were susceptible to 17 antimicrobial agents (amoxicillin/clavulanic acid, amoxicillin, azithromycin, cefoxitin, ceftazidime, ceftriaxone, chloramphenicol, ciprofloxacin, clindamycin, doripenem, doxycycline, gentamicin, linezolid, ...
متن کاملQuality controls for antimicrobial disk diffusion testing on Leptospira Vanaporn Wuthiekanun agar
BACKGROUND Disk diffusion susceptibility testing for Leptospira spp. on Leptospira Vanaporn Wuthiekanun (LVW) solid agar was reported recently. However, it was unclear whether the zone sizes obtained on LVW agar were comparable with those of other bacteria on Mueller-Hinton agar. METHODS Here, we evaluate the disk diffusion assay on LVW agar using the standard quality control (QC) bacterial s...
متن کاملMolecular Detection of Leptospira Species Serotypes in Iranian Stray Dogs
Background and Aims: Leptospirosis is a spirochetal disease with public health importance globally. This disease affects a wide range of domestic and wild animals. Dogs are one of the species most sensitive to Leptospira canicola and Leptospira icterohaemorrhagiae. The present study was concluded to evaluate the prevalence rate of Leptospira species and L. canicola and L. icterohaemorrhagiae se...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2014
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.02273-14